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Preface
1 Antibody Production by the Immune System Stefanie Sarantopoulos 2 The Antibody Molecule Stefanie Sarantopoulos 3 Antibody-Antigen Interactions Stefanie Sarantopoulos 4 Antibody Responses Stefanie Sarantopoulos 5 Selecting the Antigen Edward A. Greenfield, James DeCaprio, and Mohan Brahmandam 6 Immunizing Animals Edward A. Greenfield 7 Generating Monoclonal Antibodies Edward A. Greenfield 8 Growing Hybridomas Edward A. Greenfield 9 Characterizing Antibodies Frances Weis-Garcia and Robert H. Carnahan 10 Antibody Purification and Storage Jordan B. Fishman and Eric A. Berg 11 Engineering Antibodies James Dasch and Amy Dasch 12 Labeling Antibodies Eric A. Berg and Jordan B. Fishman 13 Immunoblotting Larisa Litovchick 14 Immunoprecipiation James DeCaprio and Thomas O. Kohl 15 Immunoassays Thomas O. Kohl and Carl A. Ascoli 16 Cell Staining Scott J. Rodig 17 Antibody Screening using High Throughput Flow Cytometry Thomas D.l. Duensing and Susan R. Watson Appendix I: Electrophoresis Appendix II: Protein Techniques Appendix III: General Information Appendix IV: Bacterial Expression Appendix V: Cautions Index |