Introduction Into the Breach xiii
Part 1 The Origins of Life
Chapter 1 Hilo 3
Chapter 2 The Gene 11
Chapter 3 DNA 17
Chapter 4 The Education of a Biochemist 29
Chapter 5 The Human Genome 37
Chapter 6 RNA 43
Chapter 7 Twists and Folds 51
Chapter 8 Berkeley 63
Part 2 CRISPR
Chapter 9 Clustered Repeats 71
Chapter 10 The Free Speech Movement Café 79
Chapter 11 Jumping In 81
Chapter 12 The Yogurt Makers 89
Chapter 13 Genentech 97
Chapter 14 The Lab 103
Chapter 15 Caribou 113
Chapter 16 Emmanuelle Charpentier 119
Chapter 17 CRISPR-Cas9 129
Chapter 18 Science, 2012 137
Chapter 19 Dueling Presentations 143
Part 3 Gene Editing
Chapter 20 A Human Tool 153
Chapter 21 The Race 157
Chapter 22 Feng Zhang 161
Chapter 23 George Church 169
Chapter 24 Zhang Tackles CRISPR 175
Chapter 25 Doudna Joins the Race 187
Chapter 26 Photo Finish 191
Chapter 27 Doudna's Final Sprint 197
Chapter 28 Forming Companies 203
Chapter 29 Mon Amie 215
Chapter 30 The Heroes of CRISPR 223
Chapter 31 Patents 231
Part 4 CRISPR in Action
Chapter 32 Therapies 245
Chapter 33 Biohacking 253
Chapter 34 DARPA and Anti-CRISPR 259
Part 5 Public Scientist
Chapter 35 Rules of the Road 267
Chapter 36 Doudna Steps In 283
Part 6 CRISPR Babies
Chapter 37 He Jiankui 299
Chapter 38 The Hong Kong Summit 315
Chapter 39 Acceptance 325
Part 7 The Moral Questions
Chapter 40 Red Lines 335
Chapter 41 Thought Experiments 341
Chapter 42 Who Should Decide? 355
Chapter 43 Doudna's Ethical Journey 367
Part 8 Dispatches from the Front
Chapter 44 Quebec 373
Chapter 45 I Learn to Edit 379
Chapter 46 Watson Revisited 385
Chapter 47 Doudna Pays a Visit 395
Part 9 Coronavirus
Chapter 48 Call to Arms 401
Chapter 49 Testing 407
Chapter 50 The Berkeley Lab 413
Chapter 51 Mammoth and Sherlock 421
Chapter 52 Coronavirus Tests 427
Chapter 53 Vaccines 435
Chapter 54 CRISPR Cures 449
Chapter 55 Cold Spring Harbor Virtual 459
Chapter 56 The Nobel Prize 469
Epilogue 477
Acknowledgments 483
Notes 487
Index 517
Image Credits 535